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      GOST 10444.7-86. Food products. Methods of detection of botulinum toxins and Clostridium botulinum

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      -GOST 10444.7-86. Food products. Methods of detection of botulinum toxins and Clostridium botulinum
      GOST 10444.7-86
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      Product details
      Document Number
      GOST 10444.7-86
      Title
      Food products. Methods of detection of botulinum toxins and Clostridium botulinum
      Document status
      Active, Most Current
      Pages count
      18
      View all details

      Product details

      Document Number
      GOST 10444.7-86
      Title
      Food products. Methods of detection of botulinum toxins and Clostridium botulinum
      Document status
      Active, Most Current
      Pages count
      18
      Published
      01.07.1987
      Delivery time
      1 business day
      Format
      Electronic (pdf/doc)
      Delivery
      download
      Description
      This standard establishes the methods for detecting botulinum toxins of all types, vegetative cells and spores of toxigenic strains of Clostridium botulinum in food products, determining the titer of botulinum toxins, serological typing of the toxin and picking of cultures of toxigenic strains of C. botulinum of the types A, B, C, D, E, F and G. The method of detecting botulinum toxin and determining its titer is based on identifying the symptoms of the clinical picture of the disease and the death of animals which are typical for the botulinum intoxication. Serological typing of the toxin C. botulinum is carried out with white mice by means of the in vivo protective test or the in vitro neutralization reaction with diagnostic anti-botulinum type-specific antitoxic serums. The method for detecting C. botulinum is based on its ability to grow and form botulinum toxins in nutrient media for anaerobic microorganisms. The cultures of toxigenic strains of C. botulinum are isolated from a product or culture fluid by inoculation on solid nutrient media, followed by the study of cultural and morphological features typical for of C. botulinum, and determining the ability of the isolated culture to form the botulinum toxin. The methods are intended: for the study of products using sanitary and epidemiological indicators; for the analysis of inoculations of products, in case of detection in them of mesophilic anaerobic microorganisms. The studies are carried out in the laboratories specified by health authorities. The terms used in this standard and their explanations are given in Appendix 1. The standard fully complies with the CMEA STANDARD 5211-65.
      ICS
      07.100.30
      SKU
      378
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