GOST 10444.7-86. Food products. Methods of detection of botulinum toxins and Clostridium botulinum
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Product details
Document Number
GOST 10444.7-86
Title
Food products. Methods of detection of botulinum toxins and Clostridium botulinum
Document status
Active, Most Current
Pages count
18
View all details
Product details
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Document Number
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GOST 10444.7-86 |
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Title
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Food products. Methods of detection of botulinum toxins and Clostridium botulinum |
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Document status
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Active, Most Current |
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Pages count
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18 |
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Published
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01.07.1987 |
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Delivery time
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1 business day |
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Format
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Electronic (pdf/doc) |
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Delivery
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download |
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Description
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This standard establishes the methods for detecting botulinum toxins of all types, vegetative cells and spores of toxigenic strains of Clostridium botulinum in food products, determining the titer of botulinum toxins, serological typing of the toxin and picking of cultures of toxigenic strains of C. botulinum of the types A, B, C, D, E, F and G. The method of detecting botulinum toxin and determining its titer is based on identifying the symptoms of the clinical picture of the disease and the death of animals which are typical for the botulinum intoxication. Serological typing of the toxin C. botulinum is carried out with white mice by means of the in vivo protective test or the in vitro neutralization reaction with diagnostic anti-botulinum type-specific antitoxic serums. The method for detecting C. botulinum is based on its ability to grow and form botulinum toxins in nutrient media for anaerobic microorganisms. The cultures of toxigenic strains of C. botulinum are isolated from a product or culture fluid by inoculation on solid nutrient media, followed by the study of cultural and morphological features typical for of C. botulinum, and determining the ability of the isolated culture to form the botulinum toxin. The methods are intended: for the study of products using sanitary and epidemiological indicators; for the analysis of inoculations of products, in case of detection in them of mesophilic anaerobic microorganisms. The studies are carried out in the laboratories specified by health authorities. The terms used in this standard and their explanations are given in Appendix 1. The standard fully complies with the CMEA STANDARD 5211-65. |
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ICS
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07.100.30 |
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SKU
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378 |